Clonal analysis of human tumors with M27 beta, a highly informative polymorphic X chromosomal probe.
نویسندگان
چکیده
منابع مشابه
Clonality and X-inactivation patterns in hematopoietic cell populations detected by the highly informative M27 beta DNA probe.
About 80% to 90% of females are informative for X-inactivation/methylation analysis with the probe M27 beta, which would therefore seem attractive in assessing clonality in hematologic cell populations. Eighteen acute lymphoid or myeloid leukemias, three chronic lymphocytic leukemias, and three chronic myelogenous leukemias as well as 12 malignant non-Hodgkin's lymphomas mostly showed extremely...
متن کاملClonality of chronic neutrophilic leukaemia associated with myeloma: analysis using the X-linked probe M27 beta.
AIMS To determine whether myeloid proliferation was monoclonal or polyclonal in a woman with chronic neutrophilic leukaemia and myeloma. METHODS The X-linked probe, M27 beta was used to determine the clonality of the neutrophil population by analysis of restriction fragment length polymorphisms and X inactivation pattern. RESULTS A polyclonal pattern of X inactivation was obtained for the n...
متن کاملCarrier detection in Wiskott-Aldrich syndrome: combined use of M27 beta for X-inactivation studies and as a linked probe.
Wiskott-Aldrich syndrome (WAS) is an X-linked immunodeficiency disorder with no clinical or immunologic abnormalities in carrier females. The defective gene has been localized to proximal Xp. Carrier females have nonrandom use of the X chromosome in granulocytes, lymphocytes, and monocytes. We have used the probe M27 beta, which detects both a variable number tandem repeat polymorphism and meth...
متن کاملA highly informative probe for two polymorphic Vh gene regions that contain one or more autoantibody-associated Vh genes.
Efforts to determine the role of specific Ig variable region (V) genes in human autoimmune responses have been hampered by the lack of suitably polymorphic probes. Recently we isolated a heavy chain V (Vh) gene, designated Humhv3005, that is 99% homologous to the 1.9III Vh gene and can encode an anti-DNA antibody. To study the relation between these two genes, different DNA fragments from the i...
متن کاملChromosomal Analysis of Tumors
In a previous study, cells from two dil'ferent pools of C3Hf mouse embryo cells were cultured in chemically defined medium supplemented with dil'ferent types of serum. The type of serum supplement inAuenced the chromosomal characteristics of the cells and also the time of their neoplastic conversion. In testing the cells for evidence of neoplastic conversion, we implanted a large inoculum of ce...
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ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1992
ISSN: 0021-9738
DOI: 10.1172/jci115733